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The present study was directed to define the triglyceride composition of pine nut oil. The triglycerides were separated from pine nut oil by thin layer chromatography, and fractionated by high performance liquid chromatography on the basis of partition numbers. Each of these collected fractions were fractionated again by gas liquid chromatography (GLC) according to the acyl carbon number of the triglyceride, and fatty acid composition of the triglyceride was also analyzed by GLC. The pine nut oil consisted of thirty two kinds of triglycerides, and the major triglycerides of pine nut oil were those of (C_(18:2), C_(18:2), C_(18:3); 34.9%), (C_(18:1), C_(18:2), C_(18:3); 10.8%), (C_(18:1), C_(18:2), C_(18:2); 9.9%), (C_(18:1), C_(18:1), C_(18:1); 6.5%), (C_(18:1), C_(18:1), C_(18:2); 6.3%), (C_(18:1), C_(18:1) C_(18:3); 4.8%), (C_(16:0), C_(18:2), C_(18:3); 3.3%), (C_(18:0), C_(18:1), C_(18:2); 2.7%), (C_(16:0), C_(18:1), C_(18:2); 2.6%), (C_(16:0), C_(18:2), C_(18:2); 2.2%), (C_(16:0), C_(18:1), C_(18:3); 1.9%), (C_(18:0), C_(18:2), C_(18:2); 1.7%), (C_(16:0), C_(18:1), C_(18:1); 1.7%), (C_(18:1), C_(18:3), C_(18:3); 1.5%).
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